Estimation of RNA by Oricinol Method | Swansea University

University
Swansea University
Course
PM134 | Microbiology
Pages

2

Academic year

2023
Author

Sanjeev K Ganesh
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0

11 EXPERIMENT NO:6 DATE: 14.11.2022 ESTIMATION OF RNA BY ORCINOL REAGENT 65 AIM 10 estimate the conc of RNA by orinol reaction 0 MATERIALS REQUIRED Standard RNA solution- 200mg/ml in IN perchloric avid/buffand saline Onimol reagent Dissolve 01g of ferric W/V chlond orinol in 100ml in of come HCT and add 3.5 ml q6% Buffered alcohol saline -0.5 7 mol/irre Nall; 0.015 md/live Nan Sodium citrate, ph PROCEDURE / Pipette out 0.0,02,04,06,08100f working Std int test tube 2. pipette out 1ml given sample in another test tube 3. Make up the volume to 1ml using distilled water The tube with 1ml distilled water all tubes is blank 4. Add 2ml orunol reagent to including blank 5 Mix the conuents by voncung/shaking what onboiling water bath for 20 min. 6. cool contents 41 reword OD at 665 nm against blank 7. plot graph with X- ans as RNA concentrational absorbance along Y-axis 8 Calmual concentration from graph. RESULTS The given unknown sample contains 64 ugRNA/ml Si

OBSERVATIONS AND CALCULATIONS volume of volume of concentration volume of Std (2004g/ml) distilled of RNA Orunol Incubate AGGS RNA water(ml) (ug) reagent (ml) in 0.0 1.0 00 boiling 2 water 0.00 butn for 0.2 0.8 40 2 20 min 0.04 0.4 0.6 80 c cool 2 0.07 0.6 0.4 120 0.15 2 0.8 0.2 160 0.17 2 1.0 0.0 200 0.25 2 1.0 unknown 0.0 64 0.08 2