Doc-Beer Lambart Law

Bue- Lamberts A . Ecl extent of absorption, absorptinity the coefficient l path length C cone of spenifie analyte sample) (absorbing medium ) Absorptimity characterizes amt of light absorbed by sperfie molecule at specific d Chromophone: / wated cow. bonded group likely 200 - 800nm The functions Cy hetero atoms having non-bonding - e pairs. Auxochrome: modifies group of atterms eg. -COOH Nth, -NH-R, OH, -SO3H * TI* a J to TI+ forbidden involves absorp two of photon of d not in UV Vis range to n to : saturated atoms, Cone pairs , less E than V to T* incident di 150-250nm.

n to TI as II to II* : insalurated provides TTe. fall in spectral region between 200-700nm t,= 1hr Bathrochromic : abs max. shifted t2 2 2hrs to longer A (ned shift) You 80 3 Hysrochronic: baseshift Hyperchammism increase in mular absorptists 26.667 Hypochromism XRD cryptallographic structure measure intensities of scattering 2s leaving material non-destructive ch tech material, waits hise tale science yyeng versatile unknown water copielline vitamins, minerals drugs, proteining DNA structural props, film thickness deviation from ideal structure How? Crystal alones scatter x-rays alim's es leatterer elastic scattering regular array of spherical waves mcgoing law destructive constructive Braggs nd 2dsmo spacing b/w diffracting planes