Brengers Method | University of California San Diego

University
University of California San Diego
Course
BIMM 100 | Molecular Biology
Pages

2

Academic year

2023
Author

Jake Tomson
Views

0

Benzer's method K. if phage in motated cant inject kcell B. if can be attacked in any case. calculate distalxe b/w afb took a b+ } cant Efect kcell. 2 phage a+ b- noplaque production a + b- - Bcell bothattack a b+ typesfrage a-b+ atb ab a+ b Allow to attack Kcell & Bcell simultaneously Rf = 2x Plaque in kcell (bcs among 2 recombireants PlaqoeinBcell only one can produceplaace) at of = a b-

h. hos ronge (hth-) Ecoli B r. Rapidlysu (r;r) Eoli B 1/2 h+ normal hort ronge affectionly B cells h affect Both B cell and B/2 cell. r+ rt progeny produce smallplaque r r progeny produce large plaque hr + b+r - Iphage attack only 1 E.coli if 2phages has attact 1 Goli, we have to add extra amoon t of phages. b- recombinate 4progenies htr- 5ir+ htr bir collected and allow to attack plate with B&B/2cell Count no: of plates h+r cloody appearance cloudy large 42 bird clear Clear small 34 plaque 12 htr+ cloody smal 12 b.r clear large Recombinents Rf 12+12 x 100 42+34+12+12 = 24%