Protein Sequencing

11 in - C - 000 weakneid I work have R a PH-3 111 CODC high coo accept H 1 from medium 1 15N- Ca - COOH charge of aa + 1 R Neutral aa 14 Nilidinocate 111 + Gall 111 liber verisling the change inplt-boffer pH 7 a amino group disociate 11+ incalle Pkaz. allow eliminated 11 from coollgroup Apillalwhich an depronate 50% of cooll group Pkas 1 OH- Pkavalue of Labove 7 L-carboryl PI . Pa pka, +Pla, sbelow: if group. (noclectricpoth 2 oneutral aa) Acidicaa- CPH 1 It caminograpterrotion I M12 11th- - cooll "--d-cco, Praz Inclebange--2 I C16-000 H CH2 - cooll Ht first in c-cco NOR - PH@whicb allsidechain cooll SH CH2 net chaig E + 1 depotement sidechair in PH@wilb Lcauboxa) OH- climinale PI = Plia, tipkar dissociate it acidic aabave 2Ptravelve 2 las thon't Phas =0. 1 phvalue>7

Yeart Sur Trof GIOL Cloa GDR, Indedol Strone Centrosome duplication PLK cinsio Robe arding I (b-daughterbud Cobi to paphase end of G. I Transcribed by Tarpathway seperation wheo, , Cln adow emilolic cjain-cola contrormal R helps In disguaction Remove white theirmativation Year smeat (seen@comps) scc113 e Entrayoles 6d as CAT-Cohoring Actimilagens (P16411) GS cyclinant @5 RS Acetyl Transpease mitogen of activate Gscydin CDR Seremis reitagent (ar) @ Endo/G2 RTK Cdc256 remove P MDK (milagen activated kinase) M CCDK NPC Dissociated intoson early Response X LateResponce Gene complexes APC+Cdb1 Lamin @saine P Gene I Frimanb H2 for , to, Myc dicidity Disassembly Protein the saylin Condensin + ofnuclear Ryand CDR4,6 themb H2A detach onego tromchromatin Rb CydinEfA condensation E25 free Nucleau memb retract to ER Cab APGree do cohensin removal Sajain Scyclin Meis 332 remove SIC / P27 PP2A @ centromere & from Gals Sgo cohesin Protection ofcohesine Centromate Cyannook Scyclin CDS peaklevel @centromere sics Acrorol P cohensin disarsemhy tic sweaksob & S OR Polokinase min CP Nucleation of microtobule SCP 00 Proteasomal degradation MTOC 7d,B,V tuboli incomplexes stabilized will augmentincomplex Sphase Cyclin- COK Dyenin 10- activation of (MG complex & Inmetazoon kineslas, to ex repliction kinosin 14 to. mitogen -) MAPK PRE kinesin 5 bipolas Actral kinetachore microlohule microtubule degadedby KPC-E3 obligase kinoin 5-0push Q Polar controomer to apolesof the microtabule cell Replication