Effective Phenotype

- mutent can grow well in lowtemp -permissive a cant grow in high lamp nonparmis temp sensitive motents function Complematation ofyeast genes can he studied X 2 X2 x 33 X, XM X5X5 X6 Xs + + + - X1 X1 + 1,0 + + - X2 X2 - 2,4 2,6 Complementation Group X3 X3 + + + 2,4,6-hurger 1,5 - Somega X4 X4 + - 4,6 3 -sanege unique X 5 X5 gone + wild 2dffingenes motantx mutant motont samegone a b C d e f a C + - + Q b + + 7 C + 4 d + + e 2 t 8

Iron- - binds to ragen in Pe' form (feroes) Perimal Hulidine N feet Part of tetrapyede ring N in monomenic form balinoglotin lock cooperative Bdd Halassonia ngoglabio- - not officity to O Y lack lack B a bacmaglatin. lowayfinity to oxygen I section of abypabilic cerve- similar tonamel enzyne 05 be kcooperativil P50 2tor o PO2 (forr) Myoglohin hanmaglobin, cooperativity, lessaffins 0.5 signoid 150 26torr P50:2toor (myoglobin)