Nuclear Magnetic Response

Nuclear magnetic Resonance when oriented nuclei are subjected to E.M radiation at the Proper frequency busing the clum N Energy absorption apilio occurs. illun evi to draman Lower energy State nuclei B & spin from flip if to the higher energy Magnetic nuelei In resonance with applied field

IIIII 11111 High energy sprflp Ho Kno law energy Exited State Ground state adutamin Absorbing RF radiation causes the nucleus to spin flip hr higher creagy DE State lower energy State Bo Types of Nuclei Chemistry- 'H, 13c. Biosciences - 'H / 13c, ISN, / 19 19F, 3' p etc Medicine "B-rotation 14,170. -1/2 rotation Instrumentation EMR NMR Components light N mag field rotatin An NMR 6 machine consists of: +1/2 rotation (1) A Powerful / Superwoled magnet (stable, with sensitive control, Producing a precise magnetic field). 2 A radio - frequency transmitter (emitting a very precise frequency)

(3) A detector to measure. the absorption of radiofrequary by the sample (4) A rewrder (to plot the output). NMR Spectroscopy The sample is dissolved n solvent in a thin NMR tube, and placed in a magnetic field. In the NMR probe, the sample is rotated in a magnetic field and irradiated with a short Pulse of RF radiation console NMR Spectrum. Radio transmitter frequency Radio frequency receiver & amplifier Sway was swapwils Magnet Control Console For and Magnet Reco, pole jei it' for to 9 is .) PI is was Swees greatest OT H! exist Key words a H L-Rotatin More i, lessenergy is ty B-rotatin. required and vice Versa Shielding office HIND Dieshielding, Console NMR. Instrument ( reinng forcissa TMs. Him Denterated Solvent

when atom a shilled with more e shilling is there so less energ only required to rotate H. (las i Eg CITH H3 More shoulded shield decorragiting so it is deholds the Around 10cm 6 Wile Depensive into luke broggion buddand lique N, -lase) rolate from x-3 - 300 Rf on super waled ragget NMR tube liquid He (-269°c) fig - Console for Sample with Sample mixedwell long gradient all Deuterated Solvents Eg Deuterated Acetone ((DD) 2 w) is a from of acetone (H) co 2 in which H atom ("H") is replaced with deuterium (heavy H) isotope (D) Deudereded acetone is a common solvent used in NMR spectroscopy 1H ions replaned with heavy by drogen mulecule)