UV-visible Spectrocopy

University
Swansea University
Course
PM134 | Microbiology
Pages

3

Academic year

2023
Author

Sanjeev K Ganesh
Views

0

law Not obeyed at high Con lone UV- Visible Spoctrosupy Basically it involves the spectroscopy of photons and spectrophetic metry H uses light in the visible and adjacent near UV and near Infraced (NIR) ranges UV/vis spectroscopy is routinely used in the quantitative determine of solns of transition metal ions and highly conjugated organi compounds d-bik For the quantitative measurements / Beer lambort law is followed Beer lambart law is uscful for characterizing many compound but does not hold as a universal relationship for the concenteration and absorption of all / substances. UV - Visible 200-400nm 40unm- 700mm. UV-C radiation used for disinfection is most effective at a wavelength of 264 nm. ( Peak gemicidal efficiency 264nm).

WA - 315 400 UVB - 280 315 UVC 200-280 Vacuum Uu 100-200 Instrumentation anthone shit Exitstel GS priyn Deter luvette Source (Sample holder) Monochromation soune of UV -H/ - /denterium lamp Source of visible light -Tungsten. Monochromator Optical device that transmits a mechanically Sate Selectable narrow band of wavelengths of light / other radiation chosen from a wider range of wave lengths available at the input. Double beam UV- Visible Spectro photometer Mim Delact Refunne Spectrometer Luvelic lers light understands Sours ) Detector Beam w will Splitte. Spectromet Sample len without At NHW with

slil entere exit slit Blask 3 Bean Splitter Shorple 4 more light is absorbed then transmittance is low. ie, wne & \ %transmiss % dominion Absurbance Conc , Cone (To know the unknown Sampl graph is plotted like this) Beam two . Splitter - Optical Device that Splits a beam of light in beam splitter Input beam transmitted beam Ryflected beam Operation /methodology Measure of absorbance of light of a single wavelength by the solvent alone and then by an experimental soln with the same wavelength.