Lecture Note
University
Swansea UniversityCourse
PM134 | MicrobiologyPages
2
Academic year
2023
Sanjeev K Ganesh
Views
0
Visible spectrophotometer Glass Chromophonic Structure Group nm Structure 280 X=O Carbonyl -N=N- 262 Azo 270 Nitro -N=0 330 Thioketone - c = S 230 Nibrite -NO2 Conjugated Diene -C=C-C=C- 233 Conjugoted Trien -C=C-C=C-C=C- 268. 315 Conjugated Tetraena -C=C-C=C-C=C-C=C- Benzene 261 UV Spectrometor Application Visible Spectrometer Application Protein Niacin it Amino acids (aromatic) pyridoxine Pantothenic Acid. Vit B12. Glucose determination. Metal Determination (Fe) Enzume Activity (He xokinasel Fat-quality Determination (TBA).
Reflectance Spectroscopy Principle IR source sample - Detector It fourses on the radiation reflected by different materials. H is used for Samples that are difficult to analyze by transmission. - The Sample can usually be analyzed as is without the need for preparation or any modification Instrumentation entwige cut hubmit light source Spectrometer 0 6881 fibre probe Legan huston to restricted
Visible Spectrometer
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