Hematology Lab - 18 Thrombin Time | Mt. San Antonio College

Academic year

2023
Author

Carlo Mananquil
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0

p {margin: 0; padding: 0;} .ft00{font-size:13px;font-family:ArialMT;color:#000000;} .ft01{font-size:12px;font-family:ArialMT;color:#000000;} .ft02{font-size:13px;font-family:Arial;color:#000000;} .ft03{font-size:12px;font-family:SymbolMT;color:#000000;} .ft04{font-size:12px;font-family:Arial;color:#000000;} .ft05{font-size:12px;font-family:CourierNewPSMT;color:#000000;} .ft06{font-size:13px;font-family:SymbolMT;color:#000000;} .ft07{font-size:13px;line-height:16px;font-family:Arial;color:#000000;} .ft08{font-size:13px;line-height:16px;font-family:ArialMT;color:#000000;} HEMATOLOGY LABORATORY THROMBIN TIME OUTLINE • Thrombin Time o Principle o Materials o Methods • LABORATORY EVALUATION OF SECONDARY HEMOSTASIS: THROMBIN TIME • Thrombin time is used to measure the availability of functional fibrinogen. TT is prolonged in cases of low levels of fibrinogen, when fibrinogen function is impaired, and in the presence of heparin, fibrin/fibrinogen products, and thrombolytic agents like streptokinase. • Fibrinogen (factor I) is a soluble plasma protein that is instrumental in the normal coagulation process. Following trauma or injury, fibrinogen is converted to an insoluble fibrin clot by two stage process. In stage one; thrombin cleaves fibrinogen to form a fibrin monomer. In stage two; these fibrin monomers aggregate to form the soluble fibrin polymers that are recognized as the end product in thrombin clotting assays. • The thrombin Time (TT) is a routine test to monitor heparin therapy. PRINCIPLE • The thrombi time is a simple test to screen conditions that can interfere with the conversion of fibrinogen to fibrin. A low potency thrombin is undiluted plasma and clot formation is timed. MATERIALS • Two way needle • Citrated evacuated tube • 0.5 ml serological pipette • 0.6 ml serological pipette • Tissue paper • Timer • Thrombin Time Reagent • 37oc water bath • Wasserman tube • Centrifuge PROCEDURES 1. Pipette into prewarmed test tube 2. Plasma/ control 0.2 ml 3. Incubate 3 minutes at 37oc 4. Add thrombin time reagent 0.1 ml 5. Start timer with addition of reagent 6. Record time required for clot formation • Reference Range : 8- 14 seconds •