Carbonic anhydrase

Elain Carbonic anhydrase - kinetically perfect Kcat - 107 lastbant, because km P2diffuse toform co factor H2CO3 Zinc Ka=2 Ka = 6 HA Ht+A- 2acids more acidic ka [H+][A-] [HA] ka more acidic ka & less acidic pka. 2 more acidic pka=6 pka -velogka pta pka low for Aspartate (stonglyacidic) Mechanism of Carbonic Anbydease decrease pka of waterfrom 15107 so it easily displace Ht H H - H H+ 2+ Zn 2+ Zn His His His His His His HCO3 decrease CO2 pka 0 H201 11 H H C !! 6 Zn2t Zn2+ His His His His His His

orteoclast. are deminentization influence calcium inblood bycoleochest activity parallygooid by deminarizing bone& promote calcilonio excrattion of cooples ionphosphate vit D3 Decrease blood Ca level. Peomote dictary absorption of calcium peomote Ca2'1 transpertes in interstine loin How exteoclast produce HCI secreation to bone matrix CO2 I H2O 1120, K 11+ HCO3: ,CIT ADP+P: ATP M type 11 CI ca2 + Bone HCI dissolvebone 5 mass extinctions. Ordonician Devonian. Pamian especially mauine Triaesic Crelaceas

Peimian tranic eluption CO2 to atm water/maine locaaD CO2+H2O H2CO3 attestive shereton in marine organism 08 Ca2+ in ERmemb skeletal muscle cardiac& smot pump PM voluntary involuntary skeletalf cardiac smooth striated non-striated Skeletal muscle contraction During develop ment myoblast fosed muscle fibre (multinucleated ceil) Individual cells of muscle cytoplasm muscle contain Myofitrils Cauanged inctitive length Repeating unit of muscles) of myofimil Z, Z Thick Thin filament filament Sarcomere actin myosin