Eukaryotic mRNa degradation

Eukauyotic MRNA degeadation De adenylation dependent (A210 no PABPC - exosome continue 3'-5' degradation mRNA linearise LSM2 Decappingenzyme @3end xrn1(5-3)

Deadenglation Independent mRNA with Blyndetroyed faulty mRNA with EJC UpfI, Decapping, Xrn't Endonudeolytic miRNA - -impafectly pair with target RNA block translation SiRNA Perfecty pair with target RNA. & cleave it MRNA degeadation occur in P bodies in Cytosot Allenzymes localizedin P bodies. BISP RISC RNA Induced silencing Complex - Pocas slicer activity. Arganof protein. Histones lack intron, PolyA tail (lack intranfor rapidsynlbesis, lay Poly A has besend highly conserved; ancient protein denot bascap, universally stable 5' 3' loop). stem floop give stability tomRins. degrachationrepire addition of Poly U 7 (20-40)

some mRNA has stabilizing Element bound by SE Protein, they prevent binding of decapping enzym es, exonomest xr11 SP Decapping, xrs1, exc stabilize MRNA SE DP Deca Enzyme, xrn1, exo DE destabilize mRNA Destabilizing Element 119