Recombinant System

RAG-J VDJ recombinase Complex RAG. 2 only in lymptocytes Recombination signal Sequence (RSS) : sequences which promote recombination aca Heplameic sequence (H) Nonamel requence (c) sequence. 12bp/23bp (N) good) Achain DNA Twotern RSS HSN one loss RSS s NSH chain DNA heavy chain DNA Heavy chain reargoment D-J joining V-DJjoining V-DJ Cy / V-DJ CS joining byalternate splicing. Poly Atail mRNA Translation Nascent polypeptide with L yheavy 8 heavy Chain chain.

Only IGM4 Hav - Jummery ) Mothers formed by Class switching Remove Cy and join Cd to it VDJCM VDJC Peexixe ofcertain cytokines 1L4 Cy 2 car CE Antibody Diversity Generation Multiple gamline gene segment. Combinatorial VDJ joining Junctional flexibility During recombination some sequences may be lost /gained, thislaeates Junctional differences P&N nucleotide addition. nucleotides at 3'end During recorobination process, we are adding somatic hypermotation Rate of motation in very high: change of individual nucleotides in V4J segment. AID Activation Induced Cytidine Deaminase cifidin uracil in induced RNA editing family enzymes. by this enzyme