DNA Staining

+ve stain adhere of Peptidoglycan Staining -ve stain adhere on LPS ethon of LPS destruct - venow expose theirpeptidoglycan layer counter stain with sefranin Toxin LPS anexample of endotoxin exotoxin -delibrately secreated toxins to kill host, neighbour normal cell et component of eg:- enderatoxin. bacteria act as Pectosis toxin toxin shiga toxin Encotoxin. NAD (formation of Adenia) B & # A (active ADP. + ribose + nicotinamide channel enter through the channel NAD source of ADP ribose group forming part Ebolera toxis AB5 ADP riborglated GL -GT- exotoxin constitutive active channel A B active channel more Nat int catalytic ADPeypoxylate to GL GDP GXGTP open Na+fo Cichand more salt to women more water to women 1 diarboea

Petrusistoxin A'B's Bordella petresis ad on respiratory back Generate cough Petrosin loxi in A B GLIGD ADPR nowin inibitory configuration more CAMP + more PKA more saltto respiratory tract Generate cough. Shigotoxins Ricin (Ricims commonis) AB5 (same mechanism) remove critical 'A' from 28SrRNA shigella B 1 Translation Diphtherialoxin AB. + Exoloxin A mechanism). A-B Crame Corenobacterium diphlberia Pseudomona aurogino ADPEgharitonyation of EF2 (wkalyetic ADP mysolyted to translocation Diphthamide (delived factor) frombirfidine) So called diphtheria toxin