Yeast sacharomyces cervisiae

8/19 Yeast Sacharomyces Celvisial haploid Diploid (Depends on nutrition) a a (when (n) (n) nutrients Sensedby switch b/w x) ToRpathway when nutrients present a + d homothalism (making System) ax (2n) Mate switching TE MGAP 4spores TEMGER bud a a 2a 20 mother nucleolos a D CDC14phosphalar M a no abong in matingtype. ribosomeausably only Allowequal abouted mother P53Clumor supressor) canswilch a&a mating to a a M a be 0 M 8 M D M D a a a a

Mating type depends of MAT locus + left and right contai n HMLX & HMRa - a & locates in end of chromoome under influenced telomere sequence (highly condensed) Silerxer making type locus (MAT) Silence exist in condensed THE & wa HMRa and transcriptionally inactive form due Left centro Matak Right totalomere influence mere Transcriptionaly inactivebes they are present near centrama - Position effect Adhomosomal invession leads to loss of a gene if f it occurs in the middle of a genere gene If it owns b/w gone, now active gene in nearthe contromere under the influence of the heleiochromatic centromele, this gone in navinactive Position effect. Switching HOE endonclease cut aportion of HMLa and remove a & place HMLK into a L mat locus cut a piece from HMRa and place to matlacs 2-'a In had, Han Asb (expressed only inhod) Synthesized in mother hotall how Ash only in bod transportest to had via actin

Sbez (adaptor) she3(another adaptor) UTR 3 5' 3 ide I mRNA mother mother produce ashmRNA Anyonin but transported to bud by myoin 5 vehicle with adaptors (she2 & sbe5) through actin Road bud 5 3' - 00000200000 + pdanized action filament. Incolture Saddition ofmyoblast ,fosed together to form muscle fibre cell, acetyicholine receptor distribute to enter PM of muscle fibre cell @ density of 20/4m ,addition of neuron to the culture helps to concentral AC R to middle of muscle cell site of necromoradal junction, ,nowdensity in 20,000/4/m3. Motor neuron axon connet aconteimines to middle cell Again secreated hypers 1 LRF4 2 laqrio bird to LRPReceptor Skdetal mede RTK on misk acctylcholine Receptor tocente. Hirougha actin road (Nust FM RIK 4 cylosol RhoGES 5 caseyacetyl 5 choti ne actionstymerization receptor Refer bioid naDo) torentre patitioning of p.melanogade ofrail with f chloroplant movement help of Rapsyn

In culture before addition of neuron, density of axetyl trost chotine receptor in muscle in 20 per prom? after addition of neuron , nerve cell sense signal again Pharmone induced maling of haploid yeast density 20,000 perm mm2 affactor 0 0 a factor receptor afactor receptor Pharmane signalling c morphageness & shmooformation dietoactin polymelization e + nutrients I 2n + -nutrients. - Nutrients. mitotic growth meinis a @ sporulation a x a a