Isolation of Bacteria Using Enrichment Method

Date 26/05/23 5 Expt. No. Page No 10 ISOLATION OF XENOBIONT DEGRADING BACTERIA BY SELECTIVE ENRICHMENT TECHNIQUES AIM To isdate renobient degeading bacteria by selective anrichment techniques PRINCIPLE Biodegradation of petroleum hydrocarbons is a complex process that depends on the nature Cy on the amount of hydrocarbons present Peledeum hydeocarbons can be divided into 4 classes : the saturates the aromatics the asphaltenes 41 the retins. The susceptibility of hydeocarbons to microbial degradation can be generally ranked as follows: linear alkanes branched alkanes small alomatic cyclic alkanes the extensive biodegradation of alkyl aronatics in marine sediments which oursed peior to detectable biodegradation of n. alkare people of rude oil 4 the micooganisms namely Arthrobacter Buskhadderia Mycobacterium Pseudamonas, sphingomonas 4 Rhodocouns were found to be invelved for alkylaromatic degradation MATERIALS REQUIRED Enrichment beath soil sample , arposed to oil, petriplates conical flask. PROCEDURE Enrichment of oil degrading bacteria:- / the soil sample exposed to oil was collected ujused for Teacher's Signature

Date Expt. No. Page No. // isdation of oil degeading bactelia 2 Enrichment loan was prepared by autoclaving the media 3 1g of soilsample was weighed 4 added to the enrichment broth aseptically alincubated in the increator to , day at 120 rpm Isolation of oil degeading bacceria : 1. the enrichment broth showing turbid bacterial growth were further speead plated on to enrichment agai which will selectively isolate the oil degrading bacteria 2. The plates were incurated at 37°C for 5 days. 3 the alony growth was observed cy maphologywas recorded. OBSERVATION Altre performing gean staining pink coloured rod shaped bacteria were observed under 100x oil immersion microrope. RESULT The isolated xenobiont degeading bacteria is gean negative bacillus Teacher's Signature