Endospore Staining

/ EXPERIMENT 5 DATE - 18/10/22 ENDOSPORE STAINING PROSSURE , Inte a lean grease see slide 4 make a imeal using steele technique 2 Au day E heat fix the aganum on the glass stide at cave with a square of blocking paper 3. Salucate the blotting paper with malachete geen stain solution up clean for 5mins keeping the paper most up adding mal dye as required on the slide may be steamed over a container of baling water 4. wash the slide with Tap water 5 counterstain with 05 % saffernin for 30 seconds wash with tapwates 4 blot day. 6 Examine the slides under mires cope for the presence of endospoll OBSERVATION Under 10x objective lons - bright geen colored spores were visible of under 100x lens the spores were even more leally visible RESULT The beight green coloured spoes confirms the presence of endospoles Hence the bacterial cells have developed endospoles DISCUSSION Bacterial endospoles all metabolically inactive, highly resistant structures produced by some baderia as a defensive strategy against unavareable envison men. tal conditions The bacteria can remain in this suspended state until condition become favourable El they can germinate at return to their vegetative state

10 Endospores are bright geen in colour y vegetative cells all brownish red to pink Spous may be located in the middle of the cell, at the end of the all , or between the end 4 middle q the all Spou shape may also be of diagnostic use. spaces may be spherical or elliptical or