Indole Test

iii INDOLE TEST AIM To access the ability of the isolate to produce type to pharase enzyme MATERIALS REQUIRED 1 Teyptone broth 2 Kovac's reagent 3. Bacterial culture PROCEDURE andde production is dependent upon the presence of typtophar in the culture medium 1 A 1% solution of peptone water is generally used in te for indole production because it is rich in Hyptoph Bacteria is to be tisled are incubated for 48- 72 ha 2. 5 drops of Kovac's reagent are added to culture broth Indole reacts with Kovac's reagant to give a cherry 3. red ring at the top surface of the test tube. A ye colour indicates a negative result OBSERVATION A yellow coloured ring was observed at the to surface of test tube

16 RESULT The given organism is indde negative DISCUSSION Indole lest is abiochemical test which differentiales the colitian from other members of Enterobacteriaceal by relecting their ability to produce the enzyme kyptophanase This enzyme hydeolysis the aminoacid tryptophan into indole / pyeuvic acid as NH3 97 is me inteacellular anyyme (endoengyme)