Citrate Test

p {margin: 0; padding: 0;} 18 V CITRATE TEST AIM To perform citiall utilization last for the detection of facial coliforns PROCEDURE / simnons citrate Agar plates well prepared ul the againsm was streaked with the help of loop 2. Alain streaking , the plates were inculated in the cubator. 3. some organisms may re quice upto 7 days of incurration 4 observe the development of blue colour OBSERVATION The plates were converted from green to blue clair RESULT The organism is found to be chale positive DISCUSSION The ulrate utilization test is a part of the IMVIC test that differentiales organisms on the basis of cacie ability to use citrate as a sole source of eneregy. The ciliate test is performed along with the other INVIC tests to differentiali Gram negative bacilli of the knlbeobacteriaceae family . It is important lest mat allows the species - level identification of members of Entero bacteriacial family. with the exception of a few spacies , Salmonella Klebrulla ) Enlero bacter usually give a positive reaction of Escherichia / shigella of Yessinia give a negative reaction Proteus is a citrate variable B simmon's citrate test as it utilizes simmon's itrate agar that contains citrate as major source of energy The medium furth wntains NHUL22PO4 as sole cource of N2 Organisms give a positive result in this left should be capable of famenting citrals in the presence of the anymo whase.