26 EXPERIMENT NO : 11 DATE 29/11/22 ISOLATION OF FUNGAL COMPONENTS FROM DIFFERENT ENVIRONMENTAL SAMPLES AIM To isolate fungal components from different environmental samples PROCEDURE Rose bengal Purpace ajal medium al czapek dox 1 agae medium in seperate conical flasks. reailize those media along with peteiplates for 20min at 2. 121°C 3. socially didule the sample up to 10-6. 4. speead each dilucion in the solidified media nubals the plates for 3 days at LOOM temp. 5. creek the plates 4 identify the colonies through LPCB 6 (Lactophenol cotton blue) staining OBSERVATION Fungal colonies were green grown feam both rhigosphere 4 non- chigosphere soil at different dilution no factor. In 10-2 dilution male no. of colonies were observed 4 with increase in dilution the no. of colonies were reduced on staining the tungal alony with LPCB, fungal spotes. hyphae , al fruiting structures well obtained , RESULT Fungal colonies / hyphae , druiting bodies al spores were observed under 10X al 40X.
27 DISCUSSION Fungi all the most divers Y E adaptable group of living organisms. The colonization density El fungal diversity can be affected by factor such as salinity / pn , temp. altitude 4 availability of nutrients. Fungal intraction with ecosystem to play as important role in the biodivessity survivability ,peopagation of productivity of plants. Funge have estallished a beneficial association with numerous hosts 4 have the capacieily to degrade environmental pollutants such as hydeocarbons al by products. Fungi cy tuns al products like extra cellular hy deolytic 4 ligner - degrading enjymes, 4 surfactants offer eco. - friendly of cost - effective strategies to address polution especially with respect to bioundeation of oil spells 4 polyaromatic hydeocarbons.