Microbial Growth

Microbial Growth (Module 2) Increase in cell no. & PO Increase in cellular constituents we can define microbial " growth "bireagy Aug size of bacteria L In fun. population a growth is looked to check the Microbial growth Multinucleated Increase by cell division Bactorial Growth Curve population growth only studied through closed system Batch Process. So bacterial growth curve iso Valid log of Born viable cells. . time. Bacterial cells can only reach upto 109. (maximum) Protozoan cells can only reach up to 10 6 algae Actino- 10 4-105

lag Phase There won't be immediate increase in the growth - Adaptation period- It try to adapt to the environment - Culture may be old. (io, ATP will be in depleted State - change in nutrients. cofactors depletion Ribosomes depletion change in environment ( R, stored in deep franze) ie, enzyme machinery will not work as in domant state.) S-TA) cell may be trijured. i metabolites eg. vit, organic acids ,aminoacids, Alcohol log Phace All organism multiply in a slightly varying timetime interval. All the cell will be both physio logically and biochemically active Multiply in a logarithmic fashion (io, exponential form). Stationary Phase Balance b/w cell growth and cell death. ic, why the growth curve become horizontal. Nutrient limitation. O2 depletion . Toxic metabolites are synthesized. ( ia, stress is there). (2° metabolites) tin the form of toxin ,antibiotic; bacterioun.

Decline / death phase Nutrient Deprivation More and more of toxic metaboli tea accumulation. Death in a logarithmic fashion. ie, why the growth curve will not become zero. Dead cells are still present there that is no Lysis of cell taking place