Role of ATP in Microorganisms Metabolism

Rote of ATP in Metabolism (naeworrege) many reans in cell are endorgonic and will not proceed toward completion. ATP drives Such reaction more to completion ATP- - high energy molecule ATP HHO ADP+P, AG" = - 7.3 kcal/mol.

ATP has a high Phosphate gp transfer potential ,10, it readily transfer its phosphate to water so ATP is ideally suited for its role as an energy currency. It is formed in energy trapping and generating processes such as photosynthesis fermentation and aerobic respiration. Vmar Vclock, Hyberbolic lionic Vinax V= Vmaxts 2 saw storteds Km whole mystro roo to ts Km Substrate conc km = the Substrate cone required by the enzyme to operate at hay its max velocity. Vmax the rate of pott formation when the enzyme is saturated with Substrate and operating as fast as possible Michaels Menten Kinetics - -The depedence of enzyme activity upon Substrate wnc. This Substrate worve fits the MM egn 8 which relate reaction velocity (v) to the Substrate wnc (s) using the maximum velocity and the Michaels constant (Km)

Enzyme activity varies greatly with changes in environmental factor 1) Substrate wns. - Substrate con are usually low within cells. At very low substrale wnc, an encyme makes pat slouty betown it seldom contacts a substrate molecule if more Substrate molecules are present ,on anzyine bird, Substrate more often, and the rean velocity (usually expressed int of the rate of pdt formation) is greater than at a lower substrate Car Rate of an anzyme Catalysed rean increases with substrate cone. Further in substrate wnc do not result ing a greater Velocity because the available enzyme molecules are binding Substance and converting it to pdt as rapidly as possible ie, enzyme is Saturated with Substrate and operating at maximal velocity (Vmax) : Resulting Substrate conc curve IS hyperbola 15ys Michaelis constant (Km) the Subshale wrs required for the enzyme to achieve hay maximal velouly - It is used as a measure of the apparent affinity of an enzyme for its substrate lower the Km value / lower the substrate wns at which an enzyme cataly we its rean.