Working of Dark Field Microscopy

How does it Work? objective Lor beattered light transcritted Dired llumination black Sample Condenser lone Sample Scatters some highs opaque light stop light Sarce Specimen is Placed on the stage of the manoscope and incandercent light from the microscop's light source is aimed at a lens beneath the Specimen This lene is called Condensor. The Condenser usually contains an aperture diaphray to control and focus light on the specimen. Then is collected by an objective lens. The objective lens magnifies the light and transmit it to an ocular lens and into the user's eye

Applications Used to visualize and study the animal cell Used to visualize and study the Plant cell. To visualize and study the morphologie of bacterial cells. To identify parasite Protozoan Such as Paranecium. Viewing stained Specimens H is best suited to viewing Stained / naturally Pigmented specimen. Advantages 1 It is very simple to use few adjustments involved while viewing the Image. 2 Some Specimen can viewed without staining 3 It can be used to view both stained and unstained Disodvantage D It cannot be used to View live specimen Such as bacteria 2 Only fixed Specimen can be viewed 3 H has low contrast 4 Use of oil immersion may distort the Image 5 The use of cover slip may damage the Spaimen.

Applications Used to visualize and study the animal cell used to visualize and study the Plant Bell To visualize and study the merphology of basterial colly To identify parasite Protozoon Such as Paranesium Viewing stained Sportmans H is best suited to vising Stained notifically Pigmested Spirmen Advantages 1 H is very simple to use few adjustments involved while viewing the Image. 2 Some Sperimen can viewed without staining 3 It can be used to view both stained and unstained Disodvantage It cannot be used to View live specimens Such as bacteria 2 Only fixed specimen can be viewed 3 H has low contrast 4 Use of oil immersion may distort the Image 5 The we of lover slip may damage the spaimen