Lecture Note
University
Swansea UniversityCourse
PM134 | MicrobiologyPages
2
Academic year
2023
Sanjeev K Ganesh
Views
0
n I chemical compounds in samples Other types include 7 polarization of light, resonant absorption radiofrequency radiation, , spin resonance. ESP (. Types flurorence spectroscopy X kay spectroscopy and crystallography. (XRD (colid ) flame spectroscopy. 1- Atomic emission spectroscopy 2. - Atome absorption 1. 3. - Atomic fluorescence Plasma emission spectroscopy Spark / are emission spectroscopy UV/VIS Spectroscopy IR spectroscopy Raman spectroscopy NMR spectroscopy Photo thermal spectroscopy. Thermal infra red spectroscopy. Mass Spectroscopy For a given material the path length and 0 Beer - -lambert law VV of sample are directly propotional to the absorbance of light Absurption of the light to the properties of the material rabirs through which the light is travelling.
The law states that there is logarithmiz dependence b/w the transmission (or transmissivity), T of light through a Substance and the product of the absorption coefficient of the Substance & and the distance the light travels through the / material (ie, the path length), l. Molar absumptivity wn A= Elc Path length cm Absorbane light absorption is directly propotional to path length and he conc of the solution. We can't measure substance with too much whour and those with less colou If core is too high we need to dilute it and use here Principle of light absorotion Ad bc In H2O I=Io a Light passes Jo eg more inform Glass call filled with cone of soln(c) [-less l As cell thickness the transmitted intensity of light(I) T=10-A adidas moon h Aou 40 04 20 02 0 0 0.2 0.4 O.L 02pathienight/
Chemical Compounds
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