Chapter 7: Fundamentals of Microbial Growth & Decontamination

CH 7: Fundamentals of microbial growth& decontamination microbial gn onth: happens very fast HOW IS bactena able to rep licate Biofilms occut in surfaces such as f/c. heartvalves. etc. Budding similar to binary fission asexual But diffis you none smaller cells (Bacters and fungi undergo budding) ( Bactena /Fungi able to create spores ,but they are used differently Bactena Harshenninments Fungi reproduction Bactenal ondospores ate strong No of thick Keratin wall they have they are resistant to alot of things Generation Time:how long It takes a cell to di indo (5mm-24hrs dependson nutnent you home available Bacteria - 4distinct Growth phasts Lag Phase decide whether they need toreplicate and If they need to make necessary proteins binary rate fission Log phase rapid expansion of butena = death stationary rhase same of bactena that are being generated, are the same as the ones dying rate Death Phaso you nenout nutrette S more bactena die that the onest bung produced. once you get to pack of log phase you can 0 longer maintain the same rate Temp remains the Same lowtem- enzym and reactions increased temp-Tg rowthrate and enzymative reactions Hightemps- will killcell. (maximum, minimal, or tinal) psychnotropis like the cold mesophites - middle knownges thermophiles- like T /hot temps audophiles like audic environments will be fund in and environments neutralophiles most PH 5-8) Fauntative Philes (Pi 9-11) Halophiles

antioxidantshup T aging obligate aerobes need 02 to summe minnerophi KS: use/tol only small ant of oxygen facultative: can use oxygen but they do Q need it can use 02 or fementation anaero bes: don't use 02 gentolerant ange robes . can be exposed to 02 but will ususe it obligate anaerobes anaerobes will most likely bein deeper tissues as opposed to tissue c brening connectin g to the outside we can categonce organisms by the way in whichthey gather encorp phototrophs vs chemotrophs mannital Saltagur - more different al for staph aerums millbegold on your mannitol salt disinfectant more harsh can pase or living PKIN D aseptic on human tissues Stenk (autoclare) -will wire out everything will even remore rimses decontamination physically renoving amt of bactena In a certain area some bactena will surviverut will 0 be enough to get you SICK Deamal reduction time know diff between thermal death time and thermal death point