Histology Revision (By Dr. Shaimaa Zaher) 1- The cell membrane can be demonstrated by which of the following? a) H & E stain b) Silver stain c) Electron microscope d) Iron hematoxylin stain 2- Which of the following components of the cell membrane has a fuzzy appearance when seen by EM? a) Glycocalyx b) Integral proteins c) Peripheral proteins d) Cholesterol molecules 3- Which of the following statements is true concerning mitochondria? a) They are non-membranous organelles . b) They are abundant in RBCs . c) They cannot divide . d) They regulate intracellular calcium concentration. 4- Which of the following statements best describes brown adipose tissue? a) It is formed of multilocular fat cells. b) It has poor blood supply. c) It is responsible for heat insulation. d) It is greatly increased in adults. 5- What is the ground substance component that connects ECM to cell surface receptors? a) Hyaluronic acid b) Glycoproteins c) Chondroitin sulfate d) Proteoglycans
6- The wall of a centriole is formed of: a) nine triplets of microtubules b) nine doublets of microtubules c) two singlets of microtubules d) bundles of microfilaments 7- Which of the following best describes plasma cells? a) They are fixed CT cells b) They have clock face nuclei c) They are phagocytic cells d) They form CT matrix 8- Which of the following structures appear basophilic when stained by H&E stain? a) Collagen fibers b) Elastic fibers c) Cytoplasm of unilocular fat cells d) Cytoplasm of fibrocytes 1 2 3 4 5 6 7 8 c a d a b a b d Enumerate types of membranous cell organelles? ✓ Cell membrane ✓ Mitochondria ✓ Endoplasmic reticulum ✓ Golgi apparatus ✓ Lysosomes Enumerate types of non-membranous cell organelles? ✓ Ribosomes ✓ Microtubules ✓ Microfilaments ✓ Intermediate filaments Enumerate components of cytoskeleton? ✓ Microtubules ✓ Microfilaments ✓ Intermediate filaments
Mention the lipid molecules found in the cell membrane? ✓ Phospholipid molecules ✓ Cholesterol molecules Mention function of integral membrane proteins? ✓ They act as: channels, carriers, receptors, and enzymes List methods of endocytosis? ✓ Pinocytosis ✓ Phagocytosis ✓ Receptor mediated endocytosis Enumerate types of connective tissue fibers? ✓ Collagen fibers ✓ Elastic fibers ✓ Reticular fibers Mention sites of mucoid connective tissue? ✓ Umbilical cord ✓ Vitreous humor of the eye ✓ Pulp of growing teeth Enumerate components of the nucleus of non-dividing cells when examined by EM. ✓ Nuclear envelope ✓ Chromatin ✓ Nucleolus ✓ Nuclear sap Mention the three dark areas of the nucleolus when seen by EM. ✓ Pars amorpha ✓ Pars fibrosa ✓ Pars granulosa Enumerate CT cells responsible for immunity and defense mechanism of the body. ✓ Macrophages. ✓ Plasma cells. ✓ Mast cells. ✓ Blood leucocytes.
Choose the suitable answers for column (A) from column (B) and write them in the tables for answers. 1 Column A Column B 1. Golgi apparatus a) Steroid hormone secreting cells 2. Mitochondria b) Oxidizing enzymes 3. Smooth endoplasmic reticulum c) Hydrolytic enzymes 4. Lysosomes d) Protein secreting cells e) ATP production f) Endocytosis Answers table 1 1 2 3 4 d e a c 2 Column A Column B 1. Iron hematoxylin a) Adipocytes 2. Silver b) Glycogen granules 3. Sudan black c) Mitochondria 4. Orcein d) Elastic fibers e) Basement membrane f) Golgi apparatus Answers table 2 1 2 3 4 c f a d 3 Column A Column B 1- Chondroblasts a) No perichondrium 2- Chondrocytes b) Articular surfaces of bones 3- Fibrocartilage c) Ear pinna 4- Elastic cartilage d) Appositional growth e) Ligamentum nuchae f) Isogenous groups Answers table 3 1 2 3 4 d f a c
4 Column A Column B 1- Loose C.T. a) Tendons 2- Elastic tissue b) Capsule of organs 3- Mucoid C.T. c) Superficial layer of dermis of skin 4- Dense regular C.T. d) Wall of large arteries e) Stroma of organs f) Umbilical cord Answers table 4 1 2 3 4 c d f a Put ( ✓ ) in front of the right statements and ( ) in front of the wrong statements: 1) Carotene is one of endogenous pigments. ( ) 2) The trans face of Golgi apparatus is the immature face that faces RER. ( ) 3) Peripheral chromatin is attached to the outer nuclear membrane. ( ) 4) Euchromatin appears as pale basophilic areas inside the nucleus. ( ✓ ) 5) Articular cartilage is covered by perichondrium. ( ) 6) Fibroblasts originate from monocytes. ( ) 7) Abundant SER characterizes protein secreting cells. ( ) 8) Basophils initiate allergic reactions. ( ✓ ) 9) Cytoplasm of plasma cells is characterized by electron dense granules. ( ) 10) Fibrocartilage is found in the intervertebral discs. ( ✓ )