Transcription State that transcription is carried out in a 3′ to 5′ direction. The RNA strand is built in the 5′ to 3′ direction while the DNA strand is read inthe 3 to 5 direction my. Distinguish between the sense and antisense strands of DNA Antisense strand: The strand of DNA that is transcribed to form an single strandof RNA. Sense strand: The strand of DNA that is complementary with the antisensestrand and the strand that is not transcribed by RNA polymerase. Explain the process of transcription in prokaryotes, including therole of the promoter region, RNA polymerase, nucleosidetriphosphates and the terminator. 1) The process of transcription is initiated when the enzyme RNA polymerase binds to the promoter site found on DNA. The promoter sitedetermines where DNA transcription starts on the DNA strands and alsodifferentiates which of the two DNA strands will be the sense or theantisense strand. 2) RNA polymerase then unwinds the DNA double-helix. It also functions as a catalyst for forming covalent bonds on the growing strand of RNAthat is being transcribed. 3) RNA nucleoside triphosphate is added to the growing RNA transcript and can only attach to the 3 end so the RNA only grows in a 3’ to 5’ direction. 4) When the RNA polymerase reaches the termination sequence of the DNA, it disassociates itself from the DNA strand and an RNA strand isreleased.
State that eukaryotic RNA needs the removal or introns to formmature mRNA. After the process of DNA transcription has taken place involving the resultantRNA strand to be formed, it needs to be modified further to produce maturemRNA. This modification involves a process called splicing. in RNA, the removal orintrons and the splicing together of the remaining exons is necessary to producemature mRNA.